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Part:BBa_K2150018:Design

Designed by: Jianyi Huang   Group: iGEM16_UCAS   (2016-10-12)


pT7+pTet+RBS+GFP+DT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 752


Design Notes

By adding a T7 promoter, we hope to achieve positive feedback in the presence of T7RNAP. We used GFP to test whether this can work before experimenting with our target gene.


Source

pT7 PCR amplified from BBa_K525998,pTet from BBa_R0040,RBS from BBa_B0034,GFP from BBa_E0040,DT from BBa_B0015

References